mouse pathogens testing  (Charles River Laboratories)

Journal: Scientific Reports

Article Title: Rank orders of mammalian pathogenicity-related PB2 mutations of avian influenza A viruses

doi: 10.1038/s41598-020-62036-5

Figure Lengend Snippet: Effects of single MPMs on mouse pathogenicity. The pathogenicity of single amino acid mutant viruses was observed by body weight loss ( A ) and mortality ( B ) of infected mice. Five 6-week-old BALB/c mice were challenged with 1.0 × 10 6 EID 50 of each virus or PBS (mock). Weight loss was observed for 14 days, and mice showing more than 20% body weight loss were regarded as dead. The average weight loss ± s.d. was measured by comparing to the initial weight of each mouse.

Article Snippet: Six-week-old female BALB/c mice (KOATEC Co. Pyeongtaek, Korea) were used for a mouse pathogenicity test (BIoPOA Co., Yongin, Korea).

Techniques: Mutagenesis, Infection, Virus

Journal: Scientific Reports

Article Title: Rank orders of mammalian pathogenicity-related PB2 mutations of avian influenza A viruses

doi: 10.1038/s41598-020-62036-5

Figure Lengend Snippet: The effect of predominant swine and human PB2 genotypes on mouse pathogenicity. Six-week-old female BALB/c mice were intranasally inoculated with 10 2 to 10 5 EID 50 of PR8-derived recombinant viruses containing major PB2 genotypes (panel A, SIB-series; panel B, HIB-series; panel C, rPR8), and MLD 50 was measured. During the period up to 14 dpi, mice that lost more than 20% of their original weight were euthanized and recorded as dead.

Article Snippet: Six-week-old female BALB/c mice (KOATEC Co. Pyeongtaek, Korea) were used for a mouse pathogenicity test (BIoPOA Co., Yongin, Korea).

Techniques: Derivative Assay, Recombinant